Foliar Ca, Sr and 87Sr/86Sr measurements on Watershed 1 at the Hubbard Brook Experimental Forest, 1999 - present
TITLE
Foliar Ca, Sr and 87Sr/86Sr measurements on Watershed 1 at the Hubbard Brook Experimental Forest, 1999 - present
PRINCIPAL INVESTIGATOR(s)
Joel Blum
University of Michigan
1100 N Univ Ave
Ann Arbor, MI 48109
USA
ABSTRACT:
This dataset includes ICP-OES analyses of Ca and Sr concentration and thermal ionization mass spectrometry analyses of 87Sr/86Sr ratios for annual samples of Watershed 1 understory foliage. Additional foliar elements were analysed and are included in the data table.
KEYWORD SET: Hubbard Brook Ecosystem Study LTER
calcium, ecosystems, foliage, forests, HBEF Watershed 1, HBR, Hubbard Brook LTER, isotopes, strontium, understory, watersheds.
KEYWORD SET: LTER Core Research Areas
disturbance, inorganic nutrients.
BEGIN DATE
1999-08-01
END DATE
2008-08-01
LOCATION
Watershed 1 is an 11.8 ha watershed extending from the weir at 488 m to the ridge at 747 m.
West bounding coordinate: -71.730866
East bounding coordinate: -71.725832
North bounding coordinate: 43.959356
South bounding coordinate: 43.952121
Elevation
Minimum: 488
Maximum: 747
(Unit: meter)
FIELD COLLECTION PROCEDURES
Fresh foliage of dominant understory species was sampled annually at three elevation bands within Watershed 1. Species included Viburnum, Wood Fern, Sugar Maple, Beech, Fir and Spruce. For most species, 3 replicates were collected. Samples from each replicate were place into a scintillation vial and returned to the laboratory for analysis.
LABORATORY PROCEDURES
Analyses of elemental concentrations were conducted using inductively coupled plasma optical emission spectrometry (ICP-OES) (PE-3300DV, Perkin Elmer, Norwalk, CT). Five- to eight-point linear calibration 24 curves had r2 values greater than 0.9999. For vegetation chemistry, one in-house standard and two High- Purity solutions (Trace Metals in Drinking Water and CRM Soil Solution A) were analyzed for quality control before and after each 10-sample sequence. For water analyses, an in-house standard and one High- Purity solution (Trace Metals in Drinking Water) were used. Analyses agreed with certified values to within +/- 5%. Strontium isotope ratios were determined using a thermal ionization mass spectrometer (TIMS, MAT- 262, Finnigan, Bremen, Germany) operated in static mode with the background measured at half mass. Digested samples in solution containing between 50 and 500 ng of Sr were evaporated, re-dissolved in 3 M HNO3 and the Sr was then separated in a quartz cation exchange column using Sr-specific resin (Eichrom, Darien, IL, USA). Between 50 and 100 ng of Sr was evaporated to dryness, re-dissolved in 1 ll HCl and loaded onto tungsten single filaments with Ta2O5 powder and 1 ll 0.3 M H3PO4. 87Sr/86Sr ratios were normalized to 86Sr/88Sr = 0.1194 to correct for instrumental mass fractionation. Between 100 and 200 isotope ratios were measured for each sample, yielding a mean 87Sr/86Sr ratio with an analytical uncertainty of less than +/- 0.000030 (+/- 2r), unless reported otherwise. Repeated measurements of NBS-987 during the period of analysis resulted in a mean 87Sr/86Sr value of 0.710237 +/- 0.000025 (+/- 2r, n = 138).
REFERENCES
  • Dasch, A., Blum, J.D., Eagar, C., Fahey, T.J., Driscoll, C.T., and Siccama, T.G. 2006. The relative uptake of Ca and Sr into tree foliage using a whole-watershed calcium addition. Biogeochemistry 80:21-41.
DATA ACCESS GUIDELINES
Data Use Policy



The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. Permission to use this dataset is granted to the Data User free of charge subject to the following terms:

1) Acceptable use. Use of the dataset will be restricted to academic, research, government or other not-for-profit professional purposes.

2) Redistribution. The data and metadata are provided for use by the Data User. The Data User will not redistribute the original Data Set or metadata to others without the explicit permission of the Principal Investigator.

3) Citation. It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User will properly attribute the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator, Year of Data Publication, Title of Dataset, Publisher, Dataset identifier.

Citation example: Holmes, R.T. 2012. Bird Abundances at Hubbard Brook (1969-2010) and on three replicate plots (1986-2000) in the White Mountain National Forest. Durham, NH. Hubbard Brook Data Archive [Database]. http://hubbardbrook.org/data/dataset.php?id=81 (23 July 2012)

4) Acknowledgment: The Data User should acknowledge any institutional support or specific funding awards referenced in the metadata accompanying this dataset in any publications where the Data Set contributed to its content. Acknowledgments should identify the supporting party, the party that received the support, and any identifying information such as grant numbers.

Acknowledgment example: Data on [topic] were provided by [name of PI] on [date]. These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station, Newtown Square, PA. Significant funding for collection of these data was provided by [agency]-[grant number], [agency]-[grant number], etc.

5) Consultation and questions. Data users are strongly encouraged to consult with the Principal Investigator(s) who collected these data for further information. Also, when appropriate, Data Users should consider including the Principal Investigator as a collaborator and/or co-author in the use of these data.

6) Notification. The Data User will notify the Principal Investigator of any publication or derivative work based on the Data Set. The Data User will also provide the Principal Investigator and/or the administrator of the Hubbard Brook Ecosystem Study with a pdf or two reprints of any publication(s) resulting from use of the Data Set.

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CONTACT PERSON

Information Manager, Hubbard Brook LTER
234 Mirror Lake Road
North Woodstock, NH 03262
USA

Phone: (603) 726-8902
Email: hbr-im@lternet.edu

Data file: w1folisotopes.txt
Description: Hubbard Brook foliar isotope measurements of understory species on Watershed 1.
Notes on Data: Original data are located at the Blum Lab, Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor.
ColumnVariableDescriptionUnitsCoded?Missing value label
1SampleSample collection numbernoneynone
2USpeciesUnderstory species samplednoneynone
3ElevElevation zone within watershednoneynone
4AgeNeedle age, applicable only to softwoodsnoneynone
5YearYear of samplingYYYYnnone
6AlmggAluminum concentration in foliagemilligramPerGramnnone
7BamggBarium concentration in foliagemilligramPerGramn
-9999
8CamggCalcium concentration in foliagemilligramPerGramnnone
9KmggPotassium concentration in foliagemilligramPerGramnnone
10MgmggMagnesium concentration in foliagemilligramPerGramnnone
11NamggSodium concentration in foliagemilligramPerGramn
-8888
12SimggSilica concentration in foliagemilligramPerGramn
-9999
13PmggPhosphorus concentration in foliagemilligramPerGramn
-9999
14RbmggRubidium concentration in foliagemilligramPerGramn
-8888
15SrmggStrontium concentration in foliagemilligramPerGramnnone
1687_86RatioStrontium 87:86 isotope ratio in foliagedimensionlessnnone
17Pct2SEStatiscal variability of 87_86Ratio data. Two standard errors, in percent.dimensionlessnnone
18CaSrgCalcium:Strontium ratio in foliage by weightgramnnone
19CaSrmolCalcium:Strontium molar ratio in foliagemolennone
20CaAlmolCalcium:Aluminum molar ratio in foliagemolennone
21BaSrgBarium:Strontium ratio in foliage by weightgramn
-9999
22BaSrmolBarium:Strontium molar ratio in foliagemolen
-9999
23CaNagCalcium:Sodium ratio in foliage by weightgramn
-9999
24CaNamolCalcium:Sodium molar ratio in foliagemolen
-9999
25AlmgLAluminum concentration in extractmilligramPerLiternnone
26BamgLBarium concentration in extractmilligramPerLitern
-9999
27CamgLCalcium concentration in extractmilligramPerLiternnone
28FemgLIron concentration in extractmilligramPerLitern
-9999
29KmgLPotassium concentration in extractmilligramPerLiternnone
30MgmgLMagnesium concentration in extractmilligramPerLiternnone
31MnmgLManganese concentration in extractmilligramPerLitern
-9999
32NamgLSodium concentration in extractmilligramPerLitern
-9999
-8888
33PmgLPhosphorus concentration in extractmilligramPerLiternnone
34RbmgLRubidium concentration in extractmilligramPerLitern
-9999
-8888
35SimgLSilica concentration in extractmilligramPerLitern
-9999
36SrmgLStrontium concentration in extractmilligramPerLiternnone
37TimgLTitanium concentration in extractmilligramPerLitern
-9999
38SamplewtWeight of sub-sample used for digestgramnnone
39TSwtTotal sample weightgramnnone
40AltSampleOriginal sample number used for foliage collected in 1999 and 2000noneynone
41NotesNotes on sample analysis or collectionnoneynone

CODES

Variable: Sample
Description:
A unique number representing a sample
Variable: USpecies
Code
Description
Maple
Sugar Maple, Acer saccharum
Beech
American Beech, Fagus grandifolia
Fern
Wood Fern, Dryopteris spinulosa
Viburnum
Viburnum alnifolium
Fir
Balsam Fir, Abies balsamea
Spruce
Red Spruce, Picea rubens
Variable: Elev
Code
Description
High
Spruce-fir zone.
Upper
Hardwood zone above the ledges, directly above and to the west of the upper rain gauge clearing.
Mid
Below the ledges and above the middle rain gauge clearing.
Low
Below the middle rain gauge clearing.
Variable: Age
Code
Description
na
not applicable; species is a hardwood
New
First year needles
Old
Second year or older needles
All
All ages combined.
unknown
Needle age not recorded
Variable: AltSample
Description:
Original sample number used for foliage collected in 1999 and 2000
Variable: Notes
Description:
Notes on sample analysis or collection

MISSING VALUE CODES
Variable
Missing Value Code
Code Explanation
Bamgg
-9999
Analysis not performed
Namgg
-8888
Below limit of detection for instrumentation
Simgg
-9999
Analysis not performed
Pmgg
-9999
Analysis not performed
Rbmgg
-8888
Below limit of detection for instrumentation
BaSrg
-9999
Analysis not performed
BaSrmol
-9999
Analysis not performed
CaNag
-9999
Analysis not performed
CaNamol
-9999
Analysis not performed
BamgL
-9999
Analysis not performed
FemgL
-9999
Analysis not performed
MnmgL
-9999
Analysis not performed
NamgL
-9999
-8888
Analysis not performed
Below limit of detection for instrumentation
RbmgL
-9999
-8888
Analysis not performed
Below limit of detection for instrumentation
SimgL
-9999
Analysis not performed
TimgL
-9999
Analysis not performed